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Up to now, 10% Fetal Bovine Serum (FBS (V/V)) was added to basal medium for the cultivation of hybridoma. For the cultivation of hybridoma cell line, CH07EO;against colon cance;serum concentration was reduced to 3 % FBS without influence on cell growth and maximum cell concentration. By the addition of cell growth promoting substances-insulin( I ), pyruvate(P), oxaloacetate(O), Pluronic F68(P) and 2-mercaptoethanol(2-ME)-to 1% FBS medium, a cell density higher than that with 1% FBS medium alone was achieved. FBS 3% medium was replaced by very cheap 2% Calf Serum(CS) medium without influence on cell growth rate and concentration. Cells grew vigorously in 0.5% CS+ IPOP medium. This composition was used during suspension culture and exhibited good viability and high specific growth rate.
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